Device

Part:BBa_M50505

Designed by: Matthew Prospero, Cami Tussie, Martin Alcaraz Jr.   Group: Stanford BIOE44 - S11   (2018-12-04)


uvrY Biosensor with luxSp2 Promoter

The sensor fluoresces based upon the presence of uvrY, with more fluorescence with increased uvrY. This device utilizes the luxSp2 promoter to promote the production of GFP upon activation; the promoter for the luxS gene can be induced through binding with UvrY, a known transcription regulator of the luxS gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 40
    Illegal EcoRI site found at 505
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 40
    Illegal EcoRI site found at 505
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 40
    Illegal EcoRI site found at 505
    Illegal BamHI site found at 714
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 40
    Illegal EcoRI site found at 505
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 40
    Illegal EcoRI site found at 505
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None